- No radioactive materials required.
- Applications – Flow cytometry or Fluorescent microscopy.
- Detects cytolytic activity at a cellular level.
- Works with multiple types of mammalian cell lines.
The most commonly used method to measure CMC/ADCC is a radioactive chromium-51 (51Cr) release assays (2). There are several disadvantages with this assay: it is expensive, difficult to load certain cell types, expensive to dispose of due to strict environmental regulations, and has high background from spontaneous release of 51Cr. With the use of flow cytometry, it is now possible to eliminate the need for radioactive material and increase the ability to quantify cytolytic activity on a single cell bases. Various groups have demonstrated that measuring CMC/ADCC activity by flow cytometry has a strong (95%) correlation with the traditional 51Cr release assay (3,4,5,6).