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aCella Cyclic AMP Cell Technology Ultra Sensitive Luminescence Kits
Cyclic AMP
aCella™ - cAMP: Cyclic AMP Chemiluminescent Immunoassay Kit


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Product code: CLACMP100
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Product Description

Key Benefits

  • Ultra-sensitive – Measures as little as < 1 fmol cAMP per sample.
  • Fast – Rapid assay, results in 2 hours.
  • Adaptable – Assay can be used with 96 or 384 – well plates.
  • Convenient – Suitable for use in multiple sample matices.

Assay Principle

The aCella – cAMP Chemilumnescent Immunoassay kit is designed to quantitatively measure cAMP present in cell lysates, plasma, urine, saliva, tissue and culture media samples. The supplied Sample Diluent will lyse cells, stabilize cAMP and stop phopshodiesterease activity. A cAMP standard is provided to generate a standard curve for the assay. The supplied Plate Primer solution is added to the wells of a coated white microtiter plate, followed by standards or diluted samples. A cAMP-peroxidase conjugate is then added to the wells.

The binding reaction is initiated by the addition of a polyclonal antibody to cAMP. After a 2-hour incubation, the plate is washed and chemiluminescent substrate is added. The substrate immediately reacts with the bound cAMP-peroxidase conjugate. The generated chemiluminescent glow signal is measured. The concentration of the cAMP in the sample is calculated, after making correction for the dilution.

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Kit contents and Long Term storage

Coater White Plastic Microfiber Plate (Coated with Donkey Anti-Sheep IgG)Part # 900142-8C
Cyclic AMP Standard, 1 Vial (125uL) cAMP at 1,500 pmol/mL in a Special Stabilizing SolutionPart # 900152-8C
Cyclic AMP Antibody, 1 Bottle (3mL) Sheep Antibody Specific for Cyclic AMPPart # 900162-8C
Cyclic AMP Conjugate, 1 Vial 50X cAMP Peroxidase Conjugate Concentrate Stock in a Special Stabilizing SolutionPart # 900172-8C
Conjugate Diluent, 1 Bottle (3mL) Diluent Containing Special Stabilizers and AdditivesPart # 900182-8C
Sample Diluent, 1 Bottle (28mL) Diluent Containing Special Stabilizers and Additives. Ready to UsePart# 900192-8C
Plate Primer, 1 Bottle (5 mL) Neutralizing Solution Containing Special Stabilizers and AdditivesPart # 900202-8C
Acetic Anhydride, 1 Bottle (2mL)Part # 900212-8C
Triethylamine, 1 Bottle (4mL)Part # 900222-8C
Wash Buffer Concentrate, 1 Bottle (30mL) 20x Concentrate that must be diluted with deionized or distilled waterPart # 900232-8C
Substrate Solution A, 1 bottle (6 mL)Part# 900242-8C
Substrate Solution B, 1 bottle (6 mL)Part# 900252-8C
Plate SealerPart# 90020