• Homogenous assay for active caspase 3/7.
• Breakthrough in cell lysis buffer and preservation of caspase activity.
• Results in a No wash one-step assay
• No need to wash out media from cell samples, just add the reagent directly to your experimental samples.
• Easy to Use: No need to make cell lysates or run western blots.
• Works with suspension and adherent cells.
• Applications - Standard Fluorescence Plate Reader

Cell Technology’s Apo 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase3/7, form the quenching DEVD sequence, the free dye excites at 488nm and emits at 515-530 nm.. As a result of a novel and proprietary Lysis Buffer System, the Apo 3/7 HTS Assay is a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.

Identification of single-domain, Bax-specific intrabodies that confer resistance to mammalian cells against oxidative-stress-induced apoptosis - Deyzi Gueorguieva, Shenghua Li, Nicole Walsh, Amit Mukerji, Jamshid Tanha, and Siyaram Pandey - The FASEB Journal 2006

Effects of Pacing Site on Left Ventricular Activation Sequences Using a Non-Contact Mapping System: Implications for Heart Failure Pacing - D. Xing, F. Devecchi, T. Staley, D. Glassman, J. Martins

Role of Shear Stress in the differential regulation of endothelial Cathepsins and Cystatin C - PhD dissertation - Manu Omar Platt

Inhibitor of Metalloproteinases 3 Regulates Resolution of Inflammation following Acute Lung Injury - Sean E. Gill, Isham Huizar, Eli M. Bench, Samuel W. Sussman, Ying Wang, Rama Khokha, and William C. Parks - American Journal of Pathology 2010;176:64-73

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6. Trends Biochem Sci 22,388 (1997)

• 1 vial Caspase 3/7 Reagent. (z-DEVD)2 Rhodamine 110 Part#4004
• 1 Bottle of Cell Lysis Buffer