- Saves valuable reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when this buffer is used.
- Increases the shelf-life of your plates. Depending on the activity of the coated protein, plates may be stored at room temperature or 2°-8°C for several months, or even years under proper conditions.
- Increases reproducibility. By using consistent reagents from a reliable source, your ELISAs may become more reproducible.
- Increases the signal of the ELISA. If the antibody or antigen is not sticking to the plate, or unwanted proteins are interfering with binding, the plate will have an overall low specific signal. CB1 may foster adsorption and reduce interference.
- Decreases background noise. If excess proteins are sticking to the plate, plate will have overall high backgrounds (a high signal overall, but a low specific signal). Universal Plate Coating Buffer may reduce interference.