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Apo Active 3PE

Apo Active 3PE Cell Technology Caspase / Apoptosis Detection
Apo Active 3PE
Antibody Specific Active Caspase 3 Detection Kit


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Product code: PAB200
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Product Description

Key Benefits

  • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
  • Applications – Flow Cytometery, Fluorescent Microscopy, or Fluorescent plate reader.
  • Yields both quantitative and qualitative results. Gives a strong positive signal.
  • Can be used in conjunction with other antibodies or stains.
  • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
  • Works with human, mouse and rat cell lines.

Assay Principle

phycoerythrin_antibody_based_caspase1Cell Technology’s APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with a variety of applications.


Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.

Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.


The endoplasmic reticulum is the site of cholestrol-induced cytotoxicity in macrophages - Feng, Yao, Li, Devlin et al - - Nature Cell Biology Vol 5, No 9, September 2003, pp 781-792

Combination chemotherapy with gemcitabine and biotherapy with opioid growth factor (OGF) enhances the growth inhibition of pancreatic adenocarcinoma - Ian S. Zagon, Jeffrey R. Jaglowski, - Cancer Chemotherapy and Pharmacology Vol 56/ No 5, Nov 2005

The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells - Sasha H. Shafikhani, Christina Morales and Joanne Engel - Cellular Microbiology Vol 10 Issue 4, pp 994-1007, Dec 2007

Monoclonal Anti–Interleukin 23 Reverses Active Colitis in a T Cell–Mediated Model in Mice - C. Elson, Y. Cong, C. Weaver, T. Schoeb, T. McClanahan, R. Fick, R. Kastelein - Gastroenterology Volume 132, Issue 7, Pages 2359-2370 (2007)

Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injury - Markus Horbelt, So-Young Lee, Henry E Mang, Nicole L. Knipe, Yoshikazu Sado, Andreas Kribben, and Timothy A Sutton - Am J Physiol Renal Physiol (July 11, 2007)

Cytotoxicity and Secretion of Gamma Interferon Are Carried Out by Distinct CD8 T Cells during Mycobacterium tuberculosis Infection - Thorbjorg Einarsdottir, Euan Lockhart, and JoAnne L. Flynn - American Society for Microbiology - Infection and Immunity October 2009, p. 4621-4630, Vol. 77, No. 10.

Converting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: Signaling pathway interactions - Anna S. Garza, Aaron L. Miller, Betty H. Johnson, E. Brad Thompson - Leukemia Research Vol 33, Issue 5, pp 717-727. May 2009


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Kit contents and Long Term storage

Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 VialPart# 10022-8C
PE Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 VialPart# 20042-8C
10X FixativePart# 3001Room Temperature