- Reduces the effects of the sample matrix and variation among samples. Pre-dilution of the samples is not necessary.
- Reduces background noise caused by nonspecific interactions between the sample matrix proteins and the plate surface.
Just pipette diluent directly onto the plate before you add samples.
A buffer is used to equalize any differences between the sample matrix (serum, plasma, urine, cell culture fluid) and the diluent is used to generate the standard curve.
EAD100 General Assay Diluent for Serum & Plasma Samples (Sandwich Format) EAD200 IgM (Positive Interference) Reducing Assay Diluent (All ELISAs) EAD300 Nonmammalian-Based Assay Diluent (Ag Down and Sandwich ELISAs) EAD400 Antigen-Down Assay Diluent (Ag Down and Sandwich ELISAs)
Cell Technology’s IgM Reducing Assay Diluent is formulated for use with problematic serum and plasma samples in most antibody sandwich ELISA formats. Proprietary additives have been included in EAD200 to reduce IgM-mediated conjugate bridging interference which can cause substantial background noise. This diluent contains proteins from goat sera to reduce nonspecific interactions that might occur between the sample matrix proteins and the plate surface. It also contains a proprietary calcium chelating agent designed to inhibit interference from complement and thrombin present in serum and plasma samples.