The loss of mitochondrial membrane potential is a hallmark for apoptosis.The APO LOGIX JC-10 Assay Kit measures the mitochondrial membrane potential in cells. In non-apoptotic cells, JC-10 exists as a monomer in the cytosol (green) and also accumulates as aggregates in the mitochondria which stain greenish orange. Whereas, in apoptotic and necrotic cells, JC-10 exists in monomeric form and stains the cytosol green. JC-10 was developed to increase solubality in aqueous buffers as compaired to its conterpart JC-1.
- Superior solubility in aqueous buffers as compared to JC-1.
- Applications – Cell permeable no wash Flow Cytometry assay.
- Add reagent directly to live cells, incubate 30-60 minutes and measure.
Fig (A). Jurkat cells were cultured with DMSO for 2 hours. Cells were then stained with JC-10 Mitochondrial Membrane Potential Detection Kit for 15 minutes and analyzed by flow cytometry.
Fig (B). Jurkat cells were cultured with staurosporine for 2 hours. Cell were then stained with JC-10 Mitochondrial Membrane Potential Detection Kit for 15 minutes and analyzed by flow cytometry.