- Cell permeable.
- No wash homogenous assay.
- Adaptable to High throughput assay platforms .
- Real time detection of NO activity.
- Applications – Flow Cytometry, Fluorescence Plate Reader, Fluorescence Microscopy
Diaminofluorescein –2 Diacetate (DAF-2DA) is a non-fluorescent cell permeable reagent that can measure free Nitric Oxide (NO) and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the diacetate groups on the DFA-2DA reagent arehydrolyzed by cytosolic esterases thus releasing FAD-2 and sequestering the reagent inside the cell. Production of nitric oxide converts the non-fluorescent dye, DAF-2, to its fluorescent triaole derivative, DAF-2T.
DAF-2T can be observed by: excitation 488nm and measuring emission at 515nm.
Fig. 1. DAF-2DA, the non-fluorescent probe, enters the cells where cytosolic esterases hydrolysis the diacetate groups, releasing the non-fluorescent DAF-2 dye. DAF-2 then reacts with NO and O2 to produce DAF-2T, its triazole fluorescent derivative.
Fig. 2. Phase Contrast and fluorescent microscopic images of rat aorta-derived vascular smooth muscular cells loaded with DAF-2DA.
Red wine consumption improves in vitro migration of endothelial progenitor cells in young, healthy individuals - Am J Clin Nutr 2010 92: 1 July 2010; 161-169
|DAF-2DA, 1 Vial (NOS200-1)||Part# 4006||2-8C|
|DAF-2DA, 2 Vials (NOS200-2)||Part# 4006||2-8C|